Journal: Scientific Reports

Article Title: Understanding the role of soluble proteins and exosomes in non-invasive urine-based diagnosis of preeclampsia

doi: 10.1038/s41598-024-75080-2

Figure Lengend Snippet: Characterization of urine and urinary exosomes isolated by BEST of PE and HC. ( A ) Representative nanoparticle tracking analysis for untreated urine and isolated urine from PE and HC, depicting particle diameter and the number of particles. ( B ) and ( C ) Comparisons of particle concentration and particle size between PE and HC. ( D ) Western blots of TSG101 and CD63 protein extracted from isolated exosomes. ( E ) and ( F ) Quantitative analysis of exosome marker TSG101 and CD63 protein. All data are presented as mean \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\:\pm\:$$\end{document} standard error and analyzed by paired t-test and independent two-tailed t-test. BEST, biologically intact exosome separation technology; PE, patients with preeclampsia; HC, healthy controls.

Article Snippet: The membranes were blocked with 5% skim milk for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies against TSG101 (NB200-112, Novus Biologicals, USA) and CD63 (NBP2-32830, Novus Biologicals, USA).

Techniques: Isolation, Concentration Assay, Western Blot, Marker, Two Tailed Test

Journal: iScience

Article Title: SARS-CoV-2 antigen-carrying extracellular vesicles activate T cell responses in a human immunogenicity model

doi: 10.1016/j.isci.2023.108708

Figure Lengend Snippet:

Article Snippet: TSG101 Antibody [4A10], Mouse , Novus Biologicals , CatNB200-112; RRID: AB_2256458.

Techniques: Recombinant, Transduction, Transfection, Expressing, Electron Microscopy, Membrane, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Binding Assay, Plasmid Preparation, Software, Sequencing